Human PPAR delta/PPARD ELISA Kit

Designed by Assay Genie for the quantitative measurement of human PPAR delta (PPARD), this sandwich ELISA kit uses a double-antibody format and a colorimetric detection method to deliver sensitive, specific results across a range of research areas. PPAR delta, also known as peroxisome proliferator-activated receptor delta (with synonyms including PPAR-delta, PPAR-beta, NR1C2, and FAAR), is described by the manufacturer as a key regulator of metabolism and energy homeostasis, playing a critical role in lipid metabolism, inflammation, and cell differentiation, making it a relevant target for studies of metabolic disorders, cardiovascular diseases, and obesity.

The assay is intended for use with human samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. According to the manufacturer, the kit has a sensitivity of 18.75 pg/mL and a working range of 31.25-2000 pg/mL. Reported linearity values fall between 82% and 103% across serum, EDTA plasma, and heparin plasma at dilution factors of 1:2, 1:4, and 1:8, while recovery ranges span 86-105% for serum (average 95%), 92-97% for EDTA plasma (average 94%), and 92-105% for heparin plasma (average 98%). Reagents are stored at 2-8°C for 12 months, and absorbance is read at 450 nm after TMB color development.