Human PPA1 PharmaGenie ELISA Kit (SBRS1009)

Pyrophosphatase (Inorganic) 1 (PPA1) is a magnesium-dependent enzyme that hydrolyzes inorganic pyrophosphate (PPi) into two orthophosphate molecules, supporting biosynthetic pathways, nucleic acid synthesis, and mitochondrial energy balance. PPA1 expression has been implicated in hepatocellular carcinoma, breast cancer, and glioma progression, where it influences cell proliferation, migration, and chemoresistance, making it a relevant biomarker in oncology research. This Assay Genie kit applies a quantitative immunoassay format to measure endogenous PPA1 protein in human samples, providing a human-specific capture antibody with negligible cross-reactivity to orthologous rat or mouse pyrophosphatases. The plate is supplied pre-coated, and the procedure follows an incubation, wash, and substrate development protocol readable on a standard microplate reader. Researchers studying cancer metabolism, mitochondrial disorders, and pyrophosphate homeostasis can use the kit to compare PPA1 levels across treated cell lines and patient-derived specimens. Unopened components should be stored refrigerated; allow plates and buffers to equilibrate to room temperature before each run, and avoid repeated freeze-thaw cycles of reconstituted standards to maintain assay precision. For in vitro research use only.