Human POU3F3 ELISA Kit (HUFI07119)

The Human POU3F3 ELISA Kit from Assay Genie enables quantitative measurement of POU3F3 (also known as BRN1 or OCT8), a POU-homeodomain transcription factor with central roles in neural progenitor cell differentiation and increasingly characterized in oncology research, including gastric and esophageal squamous cell carcinoma studies.

The kit ships in a microplate configuration with all reagents required to generate a full standard curve, including pre-coated capture plates, biotinylated detection antibodies, recombinant protein standards, and a chromogenic substrate solution. Unopened kits require refrigerated storage and protection from light to preserve antibody binding activity and substrate integrity through the stated shelf life; reconstituted working solutions should be aliquoted to minimize freeze-thaw cycling.

As a research-use-only reagent, this assay supports investigations of transcriptional regulation in developmental neurobiology and tumor biology laboratories, with the protocol offering flexibility for multiple specimen types including cell lysates. The pack includes one microplate sufficient for running standards and experimental samples in parallel, along with a detailed protocol insert and a lot-specific certificate of analysis.