Human POTE-E (POTE Ankyrin Domain Family Member E) ELISA Kit (HUFI08046)

The POTE gene family encodes proteins harboring ankyrin repeat domains that have attracted interest in oncology research because of their normally restricted tissue expression and frequent reactivation in carcinomas. This Assay Genie kit supplies a quantitative immunoassay workflow built on a pre-coated microplate, supporting biomarker profiling in human biofluid specimens. Each kit contains the capture-antibody plate, lyophilized reference standard, biotinylated detection reagent, streptavidin-HRP conjugate, wash buffer concentrate, dilution buffer, substrate solution, and stop reagent, sufficient for 96 determinations run in duplicate. Standard curve fitting covers a working range expressed in ng/mL, allowing interpolation of unknown concentrations. The recommended sample volume is 100 uL per well, with a complete incubation workflow of roughly 2.5 hours at room temperature. Unopened components should be kept refrigerated, while reconstituted standards are best aliquoted and frozen at -20°C to limit repeated freeze-thaw cycles. Researchers investigating POTE-E expression in tumor tissue cohorts, circulating biomarker panels, or comparative oncology projects will find the reagents suitable for research use only.