Human POTE-D (POTE Ankyrin Domain Family Member D) ELISA Kit (HUFI04781)

The Human POTE-D ELISA Kit from Assay Genie is a quantitative immunoassay for measuring POTE Ankyrin Domain Family Member D protein in research samples. POTE-D belongs to a primate-restricted multigene family built around ankyrin repeat domains, with detectable expression in normal prostate, ovary, testis, and placental tissues. Aberrant reactivation has been reported in multiple solid tumors, including breast, lung, and ovarian cancers, making POTE-D a candidate target for tumor biomarker discovery and tissue profiling studies.

The kit is configured for use with a standard microplate reader and includes a pre-coated plate, detection antibody, recombinant standards, and assay buffer sufficient for 96 reactions. Researchers typically load 100 uL of sample per well, run the assay in vitro, and read the chromogenic endpoint after stopping the enzymatic reaction. Reagents should be refrigerated on arrival, and the streptavidin-HRP component must be protected from light to retain activity.

Typical applications include comparing POTE-D abundance across matched tumor and adjacent normal tissue panels, screening cancer cell line models for POTE-D expression, and supporting mechanistic studies on POTE family gene regulation. The kit is intended for research use only and has not been validated for diagnostic procedures.