Human PON2/ (Paraoxonase 2) ELISA Kit

From Assay Genie, this Sandwich ELISA kit enables precise quantification of human PON2 (Paraoxonase 2), an enzyme that plays a key role in antioxidant defense by protecting cells from oxidative stress and lipid peroxidation. Dysregulation of PON2 has been linked to diseases such as atherosclerosis, Alzheimer's disease, and diabetes, making it a potential biomarker for disease research and drug development. The kit employs a colorimetric double-antibody sandwich detection format and is intended for use with human samples including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Performance specifications include a sensitivity of 0.188 ng/mL and a measuring range of 0.313-20 ng/mL. Reported linearity for serum (n=5) spans 86-99% at 1:2, 92-99% at 1:4 and 80-96% at 1:8; EDTA plasma (n=5) shows 85-103% at 1:2, 87-100% at 1:4 and 87-100% at 1:8; and heparin plasma (n=5) shows 87-100% at 1:2, 87-93% at 1:4 and 80-92% at 1:8. Recovery averages 96% for serum (range 90-105%), 97% for EDTA plasma (range 88-104%) and 96% for heparin plasma (range 86-103%).

The standard protocol involves primary incubation at 37°C for 90 minutes, biotin-labelled detection antibody binding for 60 minutes, HRP-streptavidin binding for 30 minutes, and TMB color development in the dark for 10-20 minutes, with absorbance measured at 450 nm after adding stop solution. Kits should be stored at 2-8°C for 12 months.