Human Poly ADP Ribose Polymerase (PARP) ELISA Kit

Quantify Poly ADP Ribose Polymerase (PARP) levels in human samples with this 96-assay immunoassay kit from Assay Genie. PARP enzymes catalyze the transfer of ADP-ribose units onto target proteins, a post-translational modification central to DNA damage response, chromatin remodeling, and programmed cell death pathways. Researchers in oncology, neurodegeneration, and cellular stress biology rely on PARP measurement to evaluate genotoxic effects, monitor apoptotic progression through detection of the characteristic 89 kDa caspase-cleaved fragment, and characterize cellular sensitivity to PARP inhibitor compounds in BRCA-mutated and homologous recombination-deficient models.

The kit is validated for tissue homogenates and cell lysates, accommodating specimens from cell culture experiments as well as fresh or frozen tissue preparations. Each kit provides reagents sufficient for 96 individual determinations in a microplate-based workflow, with all buffers, standards, and detection components included for the assay procedure. The protocol is intended for research use only and supports comparative studies of baseline versus treated PARP expression, time-course analyses following DNA-damaging agent exposure, and screening of pharmacological modulators of poly(ADP-ribosyl)ation activity.

Assay Genie produces this reagent under standardized quality control protocols, ensuring reproducible lot-to-lot performance. Common applications include cancer biology investigations of synthetic lethality, studies of post-ischemic neuronal injury, and analyses of inflammatory signaling pathways where PARP activity contributes to disease pathogenesis. The 96-assay microplate format allows mid-throughput experimental design without requiring specialized instrumentation beyond a standard plate reader.