Human POLR3G ELISA Kit (HUFI08613)

Assay Genie's quantitative immunoassay enables sensitive measurement of POLR3G (RNA Polymerase III Subunit G) protein in human samples. POLR3G is a subunit of the RNA Polymerase III complex, the enzyme responsible for transcribing small structural RNAs such as tRNAs, 5S rRNA, and U6 snRNA. The protein has been the focus of research into transcription regulation, immune cell differentiation, and oncology, where altered expression has been documented in certain tumor types and hematopoietic lineages.

This ELISA kit supports quantification of POLR3G levels in serum or plasma specimens collected for research applications. Sample handling follows standard laboratory protocols: collection by venipuncture into appropriate tubes, separation by centrifugation, and storage at -80 °C for long-term preservation, with repeated freeze-thaw cycles avoided to maintain analyte integrity.

Each kit provides a complete set of reagents for one full microplate, including recombinant protein standards, detection antibodies, wash buffer, and substrate solution. The assay uses a colorimetric readout measured on a standard plate reader, with quantitative results calculated against a standard curve generated from the calibrators provided. Intended for research use only, the kit supports laboratory investigations into RNA polymerase subunit biology, gene expression regulation, biomarker discovery, and in vitro studies of transcriptional machinery.