Human POLR3A ELISA Kit (HUFI07118)

This quantitative ELISA kit from Assay Genie enables researchers to measure human POLR3A protein expression in vitro for basic and translational research applications. POLR3A encodes the catalytic core subunit of RNA Polymerase III, an essential enzyme complex that transcribes tRNAs, 5S rRNA, 7SL RNA, U6 snRNA, and other small non-coding RNAs involved in translation and RNA processing. The kit supports investigations into transcription regulation, ribosome biogenesis, and the molecular pathology of POLR3A-linked hypomyelinating leukodystrophies such as 4H syndrome, which is caused by biallelic mutations in this gene. Researchers studying cellular differentiation, neural development, and disease mechanisms associated with myelin disorders can apply the kit to cell culture samples and tissue-derived lysates. Reagents are provided in vials and the assay is performed on a microplate-based workflow compatible with standard plate readers. The product is intended for research use only and should not be used for diagnostic procedures. Each kit includes sufficient reagents for 96 individual reactions, providing a practical format for medium-throughput screening studies.