Human POLR1D ELISA Kit (HUFI07124)

POLR1D encodes RNA Polymerase I Subunit D, a shared component of the Pol I and Pol III transcription machinery that drives ribosomal RNA synthesis in human cells. This quantitative immunoassay from Assay Genie supports research applications in craniofacial development disorders such as Treacher Collins syndrome, ribosome biogenesis, and nucleolar stress responses. Each kit ships as a complete microplate-based package containing pre-coated capture antibody, biotinylated detection reagent, recombinant protein standards, wash buffer, substrate, and stop solution, providing sufficient reagents for one full plate run. The protocol calls for loading 100 uL of diluted sample into each well, with absorbance measured on a standard plate reader following incubation. Unopened components should be refrigerated at 4 °C, while reconstituted standards are best aliquoted and stored frozen at -20 °C to minimize freeze-thaw degradation. Marked for research use only, this kit offers reproducible quantitative measurement of POLR1D for translational studies of transcription regulation.