Human POLI ELISA Kit (HUFI07131)

POLI encodes DNA Polymerase Iota, a Y-family translesion DNA polymerase that performs error-prone nucleotide incorporation opposite UV- and cisplatin-damaged templates and supports replication restart at stalled forks. The Human POLI ELISA Kit from Assay Genie provides an enzyme-linked immunosorbent assay workflow for quantifying POLI protein levels in laboratory research samples, supporting studies of DNA damage tolerance, mutagenesis, and tumor biology.

Supplied as a 96 Assays microplate kit, the reagents are configured for standard curve generation and parallel sample measurement across a full plate. The assay accepts clarified cell culture supernatants, tissue homogenates, and human research specimen preparations made using standard laboratory protocols, and is intended for research use only.

Downstream validation in published POLI work frequently pairs a quantitative protein readout with western blot, qPCR, or immunofluorescence to corroborate differential polymerase expression between tumor and matched normal tissues. Each kit ships with lot-specific performance data and a Certificate of Analysis to support reproducibility across runs and operators.