Human POLE3 ELISA Kit (HUFI07129)

POLE3 (DNA Polymerase Epsilon 3, Accessory Subunit) is a small histone-fold protein that pairs with POLE2 to form a heterodimer within the DNA Polymerase Epsilon complex. This accessory subunit contributes to chromatin remodeling during DNA replication and repair, and participates in leading-strand synthesis through its interactions with the catalytic POLE subunit. Aberrant POLE3 expression has been linked to genomic instability, making it a protein of interest in cancer biology and DNA damage research.

The Assay Genie Human POLE3 ELISA Kit provides a quantitative immunoassay for measuring POLE3 in human research samples. Each kit is configured as a 96 Assays microplate format and includes pre-coated wells, a recombinant antigen standard set, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent. Researchers can apply the kit to cell lysates, tissue homogenates, and other human-derived specimens to profile POLE3 levels across experimental conditions.

The assay supports in vitro research workflows in cell and molecular biology laboratories. Common downstream uses include correlating POLE3 abundance with proliferation markers, examining its modulation by replication stress or chromatin-targeting compounds, and validating expression changes observed in transcriptomic screens. The 96 Assays microplate layout allows multiple samples, standards, and controls to be run concurrently on a single plate.