Human POLD2 ELISA Kit (HUFI07138)

The Assay Genie POLD2 immunoassay targets the p50 subunit of DNA polymerase delta, the replicative polymerase responsible for processive leading- and lagging-strand synthesis as well as several DNA repair pathways. Researchers investigating replication fork stability, genome maintenance, tumor biology, or responses to genotoxic stress rely on quantitative protein-level readouts to complement transcript and activity-based studies. The kit uses human-reactive antibodies immobilised on a microplate and a reporter system read on a standard absorbance plate reader; laboratories should pair the assay with appropriate internal controls and a calibration curve generated from the supplied standards. Reagents are packed with cold packs during transit and remain stable when stored refrigerated at the temperature indicated on the package insert, with multiple freeze-thaw cycles of prepared reagents to be avoided to preserve binding activity. Each pack contains capture-antibody pre-coated wells, detection antibody, reference standards, and substrate solution sufficient for the stated number of determinations in a research-use-only workflow.