Human POLD1 ELISA Kit (HUFI07137)

The POLD1 gene encodes the catalytic subunit of DNA polymerase delta, an essential enzyme complex that performs leading-strand DNA synthesis, processes Okazaki fragments, and supports multiple DNA repair pathways including nucleotide excision repair, base excision repair, and mismatch repair. POLD1 dysfunction is implicated in Mandibular Hypoplasia, Deafness, Progeroid features, and Lipodystrophy syndrome (MDPL) and is frequently mutated in colorectal and endometrial cancers where it drives microsatellite instability.

This Assay Genie microplate immunoassay accommodates a 100 \u00B5L sample volume per determination and provides all components required to complete 96 reactions in a single run. The chromogenic substrate generates a quantifiable signal on standard plate readers, enabling researchers to measure low-abundance POLD1 in human clinical specimens. Applications span biomarker discovery in oncology, investigation of replication stress and DNA damage responses, characterization of progeroid syndromes, and translational studies correlating polymerase delta expression with genotype or treatment outcome.