Human POFUT2 ELISA Kit (HUFI07135)

POFUT2 (Protein O-Fucosyltransferase 2) is a Golgi-resident enzyme that catalyzes O-fucosylation of thrombospondin type 1 (TSP-1) repeats, a modification essential for proper folding and secretion of many extracellular matrix and signaling proteins. This Assay Genie kit enables quantitative measurement of POFUT2 protein levels across a standard curve range of 0.156-10 ng/mL, supporting investigations into congenital disorders of glycosylation, Notch signaling pathway regulation, and developmental biology research.

The assay is supplied as a 96 Assays microplate format with all reagents required to generate a standard curve, including pre-coated wells, detection antibody, recombinant standards, and substrate solution. Unopened components should be kept refrigerated with protection from light; aliquoting reagents helps minimize freeze-thaw cycles and preserve long-term enzyme activity. The protocol is for research use only, requires a minimum sample volume of 100 µL per well, and has been validated for use with human, mouse, and rat samples in vitro laboratory workflows exploring glycosylation biology and TSP-1 repeat modification pathways.