Human PNRC2 ELISA Kit (HUFI07143)

The Assay Genie PNRC2 immunoassay provides quantitative measurement of Proline-Rich Nuclear Receptor Coactivator 2, a transcriptional coactivator implicated in nuclear receptor signaling. PNRC2 participates in steroid hormone receptor activity and has been investigated in research on breast cancer tumor biology, where altered expression correlates with estrogen receptor-positive disease progression.

Provided as a research-use-only kit, components include pre-coated microplate wells, detection antibody, standard diluent, substrate solution, and stop reagent sufficient for the complete assay protocol. Researchers perform the procedure using standard laboratory incubation equipment, with absorbance readout on a compatible microplate reader. Each vial of reconstituted calibrator provides the reference needed to generate the standard curve for interpolating unknown sample concentrations.

The PNRC2 protein, also called proline-rich nuclear receptor coactivator 2, has been characterized in studies of steroidogenic factor-1 (SF-1) interaction, ERR-family receptor coactivation, and modulation of RNA polymerase II-mediated transcription. Investigators in nuclear receptor crosstalk, endocrine signaling, and oncology biomarker discovery have applied PNRC2 quantification alongside gene expression and immunohistochemistry workflows.