Human PNPT1 ELISA Kit (HUFI07141)

PNPT1 encodes the enzyme polynucleotide phosphorylase (PNPase), a 3'-to-5' exoribonuclease located primarily in the mitochondrial intermembrane space where it participates in RNA import, processing, and degradation. Researchers use this kit from Assay Genie to quantify PNPase protein levels in human samples, supporting investigations into mitochondrial dysfunction, oxidative stress responses, and autosomal recessive non-syndromic hearing loss (DFNB70), a condition linked to PNPT1 loss-of-function mutations.

The microplate-based workflow supports quantitative measurement across experimental replicates and patient cohorts, with a working detection range of 0.156–10 ng/mL and a recommended sample volume of 100 µL per well. Each kit includes pre-coated plate wells, biotinylated detection antibody, standard diluent, wash buffer, substrate solution, and stop reagent sufficient for the stated number of determinations. The assay is validated specifically for human PNPase with no significant cross-reactivity to mouse or rat orthologs. Reagents should be stored refrigerated to preserve antibody activity, and repeated freeze-thaw cycles should be avoided by aliquoting working solutions.