Human PNPO ELISA Kit (HUFI08622)

Pyridox(am)ine 5'-phosphate oxidase (PNPO) is the FMN-dependent flavoenzyme that catalyzes the terminal oxidation step converting pyridoxine 5'-phosphate and pyridoxamine 5'-phosphate into pyridoxal 5'-phosphate, the metabolically active cofactor form of vitamin B6. The Assay Genie human PNPO ELISA provides researchers with a quantitative in vitro immunoassay for measuring PNPO concentrations in research samples, supporting studies of neonatal seizure disorders caused by inborn errors in vitamin B6 metabolism, pyridoxine-responsive epilepsies, and investigations of neurotransmitter biosynthesis, gluconeogenesis, and heme production pathways where PLP serves as an essential cofactor. The kit is supplied as a 96 Assays configuration including pre-coated microplate wells, calibrated reference standards, detection antibody, substrate solution, and stop reagent. For research use only in academic, pharmaceutical, and clinical laboratory settings studying enzyme biochemistry, PNPO gene mutations, and therapeutic responses in pyridoxine-responsive metabolic conditions.