Human PNCK ELISA Kit (HUFI07156)

PNCK (Pregnancy-Upregulated Nonubiquitous CaM Kinase) is a serine/threonine kinase belonging to the calcium/calmodulin-dependent protein kinase family, encoded by the PNCK gene located on human chromosome X. This Assay Genie kit enables quantitative measurement of PNCK protein in human serum or plasma-derived specimens using a two-antibody capture workflow on a standard microplate, with absorbance-based signal detection.

The target is most strongly expressed in neuronal tissues, including the cerebellum, thalamus, and hypothalamus, where it participates in intracellular Ca2+ signaling cascades. Investigators working in neurobiology, pregnancy-associated physiology, and tumor biology (PNCK has been reported as dysregulated in breast and renal carcinomas) employ quantitative PNCK measurement to characterize kinase expression dynamics, signaling cross-talk, and potential biomarker behavior in clinical research samples.

Unopened kit components should be kept refrigerated, protected from light, to preserve antibody binding activity and reagent stability. Reconstituted standards and working detection solutions require freezing at -20 degrees C for extended storage. The microplate is supplied pre-coated with capture antibody, and the assay supports standard curve generation across a working concentration range suited to low-abundance analytes, with each vial of lyophilized standard providing reproducible calibration. For research use only.