Human PMVK ELISA Kit (HUFI07155)

The Human PMVK ELISA Kit from Assay Genie is intended for research use only and supports quantitative measurement of Phosphomevalonate Kinase, a key enzyme in the mevalonate pathway involved in cholesterol and isoprenoid biosynthesis. Researchers investigating metabolic regulation, lipid metabolism disorders, and oncogenic pathways commonly use this assay to evaluate PMVK expression levels in human-derived samples. Each kit provides sufficient reagents for 96 Assays, including a pre-coated microplate, biotinylated detection antibody, recombinant PMVK standard, and streptavidin-HRP working solution. The capture antibody is human-specific, with no significant cross-reactivity observed against rat or mouse PMVK. Recommended sample volume is 50 uL per well. Standard operating procedure involves incubation, wash steps, and a final absorbance measurement after adding stop solution. Store all reagents refrigerated; protect the standard from light and avoid repeated freeze-thaw cycles to preserve activity.