Human PMFBP1 ELISA Kit (HUFI07149)

Polyamine-modulated factor 1-binding protein 1 (PMFBP1), also annotated as STAP (sperm tail-associated protein), is a 1007-amino-acid scaffold component of the human sperm head-tail coupling apparatus (HTCA), where it forms a sandwich-like structural complex with SUN5 and SPATA6 that anchors the basal body to the sperm nuclear envelope. Biallelic loss-of-function mutations in PMFBP1 on chromosome 16q22.2 disrupt this junction and produce type II acephalic spermatozoa syndrome (ASS), a severe male-infertility phenotype defined by complete head-tail detachment and globozoospermia-like features. The Assay Genie Human PMFBP1 ELISA Kit lets investigators studying non-obstructive azoospermia, MMAF patient cohorts, or germ-cell developmental genetics quantify circulating PMFBP1 in clinical case-control sample sets. The kit is supplied in a 96 Assays format with reagents sufficient for 96 individual determinations, and components should be stored refrigerated at 4 degrees C between uses to preserve antibody activity across multiple runs. The plate-based colorimetric readout is compatible with standard microplate readers, and the resulting absorbance values support comparative quantification of PMFBP1 levels across patient populations. For research use only.