Human PMCHL1 (Putative pro-MCH-like protein 1) ELISA Kit

Designed for the quantitative measurement of human Putative pro-MCH-like protein 1 (PMCHL1), this Sandwich ELISA Kit from Assay Genie uses a double-antibody format with TMB substrate and absorbance readout at 450 nm. It is intended for use with human serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and the kit components are stored at 2-8°C for 12 months.

Validated linearity in serum ranges from 86-105% across dilution steps, with EDTA plasma at 82-101% and heparin plasma at 84-99%. Recovery averages 94% in serum (range 88-101%), 95% in EDTA plasma (range 89-98%), and 98% in heparin plasma (range 92-102%). A second manufacturer listing reports detection at 78-5000 pg/mL with sensitivity of 42 pg/mL, intra-assay CV of 4.6% and inter-assay CV of 7.8%, and recovery averages of 88% in serum (range 82-94%) and 90% in plasma (range 84-96%). PMCHL1 belongs to the melanin-concentrating hormone family, mapped to chromosomal location 5p14.3 (UniProt Q16048), with a molecular weight of 9,715 Da, and the assay recognises natural and recombinant human protein. The kit is for research use only.