Human PLGF ELISA Kit

Designed for the quantitative measurement of human Placental Growth Factor (PLGF), this Sandwich ELISA kit from Assay Genie uses a double-antibody, colorimetric detection method with a sensitivity of 9.375 pg/mL and an assay range of 15.625–1000 pg/mL. The kit is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and the reagents are intended to be stored at 2–8°C for up to 12 months.

The workflow is built around a pre-coated microplate. Standards and appropriately diluted samples are added to the wells and incubated, allowing the target analyte to bind to the immobilised capture antibody. After washing, a biotinylated detection antibody specific to a different epitope of the protein is introduced to form a sandwich complex, followed by HRP-conjugated Streptavidin and TMB substrate to generate a colour change. The reaction is stopped with an acidic solution and absorbance is read at 450 nm; signal intensity is directly proportional to PLGF concentration and is quantified against a standard curve.

Reported linearity falls within approximately 85–103% across 1:2, 1:4 and 1:8 dilutions of serum, EDTA plasma and heparin plasma, and recovery ranges between 87–104% with averages of 93–98% across the same matrices.