Human PLEK (Pleckstrin) ELISA Kit (HUFI07178)

Assay Genie provides this human PLEK (Pleckstrin) quantitative immunoassay kit configured as a ninety-six determination microplate format. Pleckstrin (PLEK) is a platelet and leukocyte protein that functions as the principal substrate for protein kinase C and contributes to cytoskeletal reorganization, secretion, and membrane ruffling in hematopoietic cells. Researchers investigating thrombocyte signaling, leukocyte adhesion, or inflammatory disorders can use this matched-antibody pair ELISA to quantify PLEK concentrations in blood-derived specimens collected from human donors. The kit ships with a pre-coated microplate, assay diluent, wash buffer, detection antibody, recombinant standards, and chromogenic substrate solution in a complete ready-to-use package. Reconstituted reagents should be stored refrigerated; standard curves are generated from serial dilutions of the supplied calibrators. Read absorbance on a standard plate reader and interpolate sample concentrations from the standard curve. The workflow is compatible with downstream applications including western blot validation and cell culture-based investigations of megakaryocyte differentiation. Each kit contains sufficient reagents for ninety-six wells in a single-use disposable microplate configuration.