Human Platelet Derived Growth Factor Subunit A (PDGFA) ELISA Kit

The Human PDGFA ELISA Kit from Assay Genie enables quantitative measurement of Platelet Derived Growth Factor Subunit A in human research samples, supporting studies in fibroblast proliferation, smooth muscle cell biology, angiogenesis, wound healing, and tumor microenvironment research. As a ligand for PDGF receptor-alpha, PDGFA drives mitogenic signaling in mesenchymal-derived lineages and is a target of interest in fibrotic disease, glioblastoma, and atherosclerosis investigations.

The protocol uses a 100 uL sample volume per well, reducing the burden on limited or precious specimens. Standards and unknowns are processed in parallel using a biotinylated detection antibody and streptavidin-HRP conjugate to produce a measurable color response that scales with analyte concentration. The kit is intended for research use only, and the supplied reagents should be refrigerated between uses to preserve antibody binding activity across multiple freeze-thaw cycles.

Researchers investigating PDGF signaling, growth factor secretion, stromal remodeling, or oncogenic transformation will find this immunoassay a reliable tool for quantifying endogenous human PDGFA across cell culture supernatant, tissue homogenate, and biofluid sample types.