Human Platelet Derived Growth Factor D (PDGFD) ELISA Kit

PDGFD (Platelet Derived Growth Factor D) is a secreted homodimeric growth factor ligand that activates PDGF receptor-β signaling, driving angiogenesis, fibroblast proliferation, and extracellular matrix remodeling in both normal wound repair and pathological settings. Investigators in oncology, nephrology, and cardiovascular research routinely quantify circulating PDGFD concentrations as a biomarker of tumor microenvironment activity, renal interstitial fibrosis, and vascular injury response. This Assay Genie kit is supplied in a microplate format with all reagents, calibrated standards, buffers, and TMB substrate needed to complete the run, and it accepts a 100 µL sample volume from serum-based or plasma-based specimens, urine, and cell culture supernatant for in vitro quantification. Capture antibody-coated plates and concentrated detection reagent should be stored refrigerated between sessions, while the standard curve material and streptavidin-HRP conjugate are best aliquoted and frozen at -20 °C for long-term stability. The plate itself is single-use to prevent inter-assay contamination.