Human Plasminogen Activator Inhibitor 1 (PAI1) ELISA Kit

Fibrinolysis research and biomarker discovery benefit from reliable quantification of human PAI-1, a serine protease inhibitor encoded by SERPINE1 and implicated in thrombotic, metabolic, and fibrotic disease pathways. This Assay Genie ELISA kit enables sensitive measurement of PAI-1 across diverse experimental contexts, supporting cardiovascular, metabolic, and oncology investigations.

The microplate-based immunoassay provides an absorbance readout compatible with standard plate readers. Each kit includes:

• Precoated plate
• Detection antibody
• Lyophilized standards
• Substrate solution
• Wash buffer and assay diluent

Sufficient reagents are provided for 96 assays. Researchers can process cell lysates, tissue homogenates, cell culture supernatants, and various clinical research samples in parallel.

Storage requirements include refrigeration of unopened components; reconstituted standards should be aliquoted and frozen to limit freeze-thaw cycles, while substrate working solution must be protected from light during incubation. The complete protocol fits within a typical working day for laboratory workflows.

This product is intended for research use only and is validated for human sample reactivity. Assay Genie provides technical support for protocol optimization and troubleshooting to ensure reproducible performance across experimental batches.