Human Plasminogen Activator ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit enables the precise quantification of plasminogen activator levels in human biological samples. Plasminogen activators are key enzymes involved in the regulation of blood clot dissolution and fibrinolysis, and their dysregulation has been implicated in diseases such as thrombosis, cardiovascular disorders, and inflammatory conditions.

The double antibody sandwich format uses colorimetric detection and is suitable for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Sensitivity is reported as 0.188 ng/mL, with a working range of 0.313–20 ng/mL. Storage is at 2–8°C for 12 months. Linearity at 1:2, 1:4 and 1:8 dilutions was 85–104%, 92–97% and 89–105% for serum (n=5); 92–97%, 90–101% and 83–100% for EDTA plasma (n=5); and 83–96%, 83–94% and 84–100% for heparin plasma (n=5). Recovery was 91–102% (average 95%) for serum (n=5), 85–101% (average 93%) for EDTA plasma (n=5) and 90–104% (average 99%) for heparin plasma (n=5).

The procedure consists of reagent equilibration, a primary incubation at 37°C for 90 minutes, detection antibody binding at 37°C for 60 minutes, HRP-Streptavidin binding at 37°C for 30 minutes, TMB substrate development in the dark for 10–20 minutes, and a stop reaction with absorbance read at 450 nm.