Human Placenta growth factor (PGF) ELISA Kit

A colorimetric Sandwich ELISA from Assay Genie is designed for the quantitative measurement of Placenta Growth Factor (PGF) in human samples. The assay is reactive with human samples and has been validated for use with serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Performance characteristics include a detection range of 31.2-2000 pg/mL and a sensitivity of 15 pg/mL. Intra CV is 7.1% and Inter CV is 10.2%. Linearity has been demonstrated for serum (1:2: 111-120%; 1:4: 105-114%; 1:8: 100-109%; 1:16: 93-103%), EDTA plasma (1:2: 92-101%; 1:4: 111-120%; 1:8: 115-127%; 1:16: 90-102%) and heparin plasma (1:2: 115-127%; 1:4: 101-114%; 1:8: 101-110%; 1:16: 85-95%). Average recovery is 97% in serum (91-103%) and 99% in plasma (93-105%). The assay specifically recognizes natural and recombinant human Placenta growth factor.

Placenta Growth Factor (PGF) is a member of the PDGF/VEGF growth factor family and is active in angiogenesis and endothelial cell growth, where it stimulates the proliferation and migration of endothelial cells. It binds to the receptor FLT1/VEGFR-1, and isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. PGF also promotes cell tumor growth. The native protein exists as a disulfide-linked antiparallel homodimer and can also be found as a heterodimer with VEGFA/VEGF, and is a secreted protein. For research use only.