Human PLA2G2A PharmaGenie ELISA Kit (SBRS0996)

Phospholipase A2 Group IIA (PLA2G2A) is a secreted lipolytic enzyme implicated in inflammatory cascades, antimicrobial defense, and lipid mediator biosynthesis, making it a frequent target in cardiovascular, oncological, and sepsis research. Assay Genie's human PLA2G2A PharmaGenie ELISA Kit provides a quantitative immunoassay tool for measuring this 16 kDa phospholipase across multiple human specimen types. The plate is configured for 96 individual determinations using an enzymatic readout and is validated for use with cell culture supernatants, EDTA plasma, heparin plasma, and clotted blood serum fractions from human donors.

PLA2G2A expression rises sharply during acute-phase responses and has been investigated as a biomarker in prostate and gastric cancer, rheumatoid arthritis, atherosclerosis, and bacterial infection models. Researchers tracking circulating PLA2G2A concentrations depend on reproducible antigen capture and consistent calibration standards to monitor disease progression or therapeutic response. The plate-based workflow incorporates standard incubation, washing, and substrate development steps that fit into routine laboratory schedules without specialized equipment beyond a microplate reader capable of measuring absorbance in the visible spectrum.

Each kit is intended for research use only and should be stored refrigerated between runs to preserve antibody binding activity and horseradish peroxidase conjugate stability. Reagents are supplied in vials and the kit provides enough pre-coated wells for 96 assays, supporting medium-throughput screening campaigns in inflammation, oncology, and cardiovascular biomarker studies.