Human PKC iota ELISA Kit (HUFI07199)

PKC iota (PRKCI) is an atypical protein kinase C isoform expressed in human epithelial and neuronal tissues, where it regulates cell polarity, cytoskeletal dynamics, and insulin signaling through downstream effectors such as Par-3 and Par-6. This Assay Genie quantitation kit enables researchers to measure endogenous PRKCI levels, supporting studies in oncology, diabetes research, and investigations of epithelial-mesenchymal transition. The microplate is pre-coated with a PRKCI-specific capture antibody that binds the target from diluted biological matrices; bound analyte is then revealed with a biotinylated detector antibody and streptavidin-HRP, yielding absorbance proportional to PKC iota concentration. Compatible with automated plate washers and standard laboratory workflows, the reagents remain stable through routine bench handling when refrigerated at 4°C and aliquoted to limit freeze-thaw cycles. Each kit supports the analysis of standard curves and replicate samples within a single experimental run, providing a complete workflow for quantifying PRKCI expression in translational research programs.