Human PITX1 ELISA Kit (HUFI07194)

The PITX1 protein is a paired-like homeodomain transcription factor essential for posterior hindlimb specification during embryonic development, and quantitative measurement of its expression supports research into clubfoot etiology, Liebenberg syndrome, and oncology pathways where PITX1 acts as a tumor suppressor in lung and prostate tissues.

This Assay Genie kit ships as a complete microplate-format set with pre-coated wells, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, chromogenic substrate, stop solution, and recombinant protein standards. Store unopened components at 4 degrees C; avoid repeated freeze-thaw cycles of the standard stock and keep reconstituted reagents on ice during the working session.

Researchers in developmental biology, orthopedic disease modeling, and cancer biomarker discovery use this assay to compare PITX1 levels across patient cohorts, cell line panels, and genetically modified model systems, supporting both in vitro cell culture work and primary tissue analysis.