Human PIPOX (Peroxisomal sarcosine oxidase) ELISA KIT (HUFI08105)

Built around the peroxisomal oxidoreductase encoded by the PIPOX gene, also called L-pipecolate oxidase, this quantitative immunoassay from Assay Genie enables researchers to measure native human PIPOX protein in research samples. PIPOX localizes to the peroxisomal matrix and contributes to L-pipecolate catabolism while also generating sarcosine as a metabolic intermediate, making accurate quantification valuable for studies of peroxisomal biogenesis disorders and one-carbon metabolism research.

The kit is supplied in an assay-ready configuration with pre-coated microplate wells, lyophilized standards, biotinylated detection antibody, dilution buffer, substrate, and stop solution packaged in individually sealed vials to preserve reagent activity. Researchers carry out quantitative detection through sequential incubation and washing steps performed at room temperature, and absorbance readout on a conventional plate reader provides data for in vitro studies, cultured cell lysate profiling, and tissue extract analysis.

This Assay Genie product supports laboratory work on peroxisome biology, including investigations into pipecolic acid pathway dysfunction, hepatic and renal peroxisomal enzyme expression, and the relationship between sarcosine levels and downstream metabolites. Researchers aliquot the standard, detection antibody, and working substrate immediately upon receipt to maintain consistent performance across multiple runs, and the kit components are intended for research use only.