Human PIP/Prolactin-inducible protein ELISA Kit

A high-quality sandwich ELISA from Assay Genie designed for the quantitative detection of human PIP (Prolactin-inducible protein) using a colorimetric double-antibody method. The assay is intended for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with reactivity confirmed for human specimens.

Performance characteristics reported by the manufacturer include a sensitivity of 18.75 pg/mL and a measuring range of 31.25-2000 pg/mL. Reagents should be stored at 2-8°C for 12 months. The protocol follows a six-step procedure involving reagent and TMB substrate equilibration, primary incubation at 37°C, biotin-labelled detection antibody binding, HRP-Streptavidin (SABC) binding, TMB color development in the dark for 10-20 minutes, and absorbance reading at 450 nm immediately after addition of stop solution.

PIP, also referred to as angiogenin 4, has been implicated in cell proliferation, differentiation, and angiogenesis, and is associated with breast cancer and other hormone-related conditions. The kit is therefore positioned as a research tool for investigating the role of this protein in health and disease.