Human PIH1D1 ELISA Kit (HUFI07214)

The PIH1D1 target protein is an approximately 32 kDa nucleolar component of the R2TP cochaperone complex, partnering with RPAP3 and the RUVBL1/RUVBL2 ATPases to drive assembly of small nucleolar ribonucleoproteins and maturation of the 40S ribosomal subunit. Researchers investigating ribosome biogenesis, RNA polymerase I regulation, and the heat-shock chaperone network rely on this Assay Genie kit for quantitative measurement of PIH1D1 abundance in human specimens. Each pack contains a pre-coated microplate, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, concentrated wash buffer, and TMB substrate sufficient for 96 individual reactions. Reconstituted standards and diluted samples are added to the wells, signal is developed with the chromogenic substrate, and absorbance is read on a standard microplate reader. Unopened kit components should be refrigerated; protect the TMB substrate from light and allow all reagents to reach room temperature before running the assay. Intended for research use only, not for diagnostic procedures.