Human PIAS3 ELISA Kit (HUFI07229)

The PIAS3 protein serves as a SUMO E3 ligase and a transcriptional coregulator that modulates STAT3 signaling pathways in human cells. Researchers investigating cytokine-mediated signal transduction, inflammatory cascades, and oncogenic transformation pathways frequently require quantitative measurement of PIAS3 expression levels in experimental specimens.

This immunoassay from Assay Genie enables measurement of human PIAS3 protein levels in research samples, supporting investigations into STAT3-regulated gene expression, SUMOylation mechanisms, and innate immunity. The kit provides reagents sufficient for 96 individual determinations per plate, suitable for medium-throughput screening of experimental cohorts. Each kit includes a pre-coated microplate along with detection antibodies, recombinant standards, and assay buffers required to generate standard curves for quantifying PIAS3 concentrations across experimental conditions.

PIAS3 plays essential roles in nuclear receptor signaling, p53 pathway regulation, and modulation of inflammatory cytokine responses. Its expression patterns are studied in cancer biology research, autoimmune disease investigations, and cellular stress response studies. The kit supports western blot validation work, immunohistochemistry follow-up studies, and PCR-based gene expression analysis comparing protein-level findings with transcript-level data. Researchers performing cell culture experiments and in vitro signaling studies find PIAS3 quantification useful for characterizing STAT3 pathway activity under pharmacological intervention or gene knockdown conditions.