Human PI4K2B ELISA Kit (HUFI07222)

The PI4K2B target is Phosphatidylinositol 4-Kinase Type 2 Beta, a lipid kinase anchored to membranes via palmitoylation that catalyzes phosphorylation of phosphatidylinositol at the 4-position. It participates in membrane trafficking, endosomal sorting, and Wnt signaling regulation, and has emerged as a biomarker candidate in hepatocellular carcinoma and prostate cancer research. Assay Genie supplies this kit as a research use only reagent set containing a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, calibrated standards, and wash buffer. Standard curves are generated using recombinant human PI4K2B reference material to support quantitative analysis across experimental cohorts. Researchers commonly pair this ELISA with orthogonal validation methods such as western blot to confirm expression changes in cell lysates and tissue homogenates. Each kit contains sufficient reagents for 96 individual determinations, and the HRP working solution should be protected from light during the protocol. The kit ships refrigerated and components should be equilibrated to room temperature before initiating the assay.