Human PI4K2A ELISA Kit (HUFI07233)

Assay Genie's quantitative immunoassay kit targets phosphatidylinositol 4-kinase type II alpha (PI4K2A), a lipid kinase that catalyzes phosphorylation of phosphatidylinositol at the D-4 position of the inositol ring. The enzyme is anchored to membranes via palmitoylation and contributes to phosphoinositide signaling, endosomal sorting, and membrane trafficking in eukaryotic cells.

For research laboratories investigating lipid metabolism, receptor signaling, and oncology, the kit provides absorbance-based quantification on a microplate reader. Validated human blood-derived specimens support translational studies on lipid pathway dysregulation in cancer, metabolic disease, and neurological disorders, where PI4K2A expression has been linked to Wnt signaling, EGFR trafficking, and leukemia cell proliferation.

Each kit is supplied with a pre-coated microplate, biotinylated detection antibody, recombinant protein standards, streptavidin-HRP conjugate, substrate solution, wash buffer, and stop reagent. The assay is intended for in vitro research use only, and unused components should be refrigerated between uses while reconstituted standards are best kept frozen in single-use aliquots to preserve activity. Researchers working on phosphoinositide biology, drug target validation, and biomarker discovery can apply this kit to quantify PI4K2A protein levels across experimental and clinical sample sets, and the 96-assay plate format supports medium-throughput screening campaigns.