Human PI3 Kinase p110 beta/PIK3C beta ELISA Kit

Quantitative measurement of PI3 Kinase p110 beta, the catalytic subunit encoded by PIK3CB, supports research into phosphoinositide signaling, insulin pathway biology, and oncology target validation. The Assay Genie kit supplies reagents for 96 determinations using human research samples, with a microplate format that fits standard plate readers.

PI3K p110 beta operates as a Class I PI3K catalytic subunit, converting PIP2 into phosphatidylinositol (3,4,5)-trisphosphate to drive AKT pathway activation. Researchers apply this ELISA to quantify protein expression in tumor lysates, cell line extracts, and to study insulin signaling dysregulation, receptor tyrosine kinase crosstalk, and therapeutic response profiling.

Each kit contains a pre-coated microplate, detection antibodies, recombinant standards, and assay buffer reagents. Absorbance is captured on a standard plate reader and analyzed with four-parameter logistic curve fitting for accurate interpolation across the working range.

Storage guidance: keep sealed components refrigerated between uses; allow plates and reagents to equilibrate to room temperature before each run. Repeated freeze-thaw cycles of the standard and antibody vials should be avoided to preserve binding activity and assay reproducibility.

Intended for research use only. In vitro applications only; not for diagnostic or therapeutic use.