Human PHRF1 (PHD and RING finger domain-containing protein 1) ELISA KIT (HUFI04976)

This quantitative immunoassay from Assay Genie enables researchers to measure endogenous levels of PHRF1, a ubiquitin-fold modifier 1 (UFM1) pathway component harboring both PHD and RING finger domains. PHRF1 plays a critical role in the cellular DNA damage response, particularly in non-homologous end joining (NHEJ) repair, making this kit valuable for oncology, genotoxicity, and DDR signaling investigations. The microplate-based workflow uses a pre-coated capture antibody plate with detection antibody and streptavidin-HRP reagents supplied in separate vials. Reconstituted standard should be aliquoted and stored frozen at -20°C to preserve activity; the kit is intended for research use only and supports cell lysate and tissue homogenate studies. The protocol requires a 100 µL sample volume and delivers results on a single microplate run. Each kit includes a sealed foil pouch containing the pre-coated plate, lyophilized standard, detection antibody concentrate, dilution buffers, wash concentrate, TMB substrate, and stop solution.