Human Phosphotyrosine TrkB PharmaGenie ELISA Kit (SBRS2004)

The Phosphotyrosine TrkB PharmaGenie ELISA from Assay Genie is a phosphorylation-specific immunoassay engineered to quantify tyrosine-phosphorylated TrkB receptor in cell and tissue lysates. TrkB is the high-affinity receptor for brain-derived neurotrophic factor (BDNF), and its activation through autophosphorylation at tyrosine residues triggers downstream PI3K/Akt, MAPK, and PLCγ signaling cascades that govern neuronal survival, synaptic plasticity, and memory consolidation. By capturing only the phosphorylated form of the receptor, this ELISA enables researchers to directly measure ligand-induced TrkB activation rather than total receptor expression, supporting studies in neuropharmacology, depression research, neurodegenerative disease modeling, and stem-cell-derived neuron differentiation. The assay is calibrated for human samples with a human-specific capture antibody, minimizing cross-reactivity with rodent TrkB orthologs. Standard curves are generated using serial dilutions of the provided phospho-TrkB reference, and absorbance is read on a standard microplate reader. Reconstituted standards and detection reagents should be stored at -20°C with repeated freeze-thaw cycles avoided by aliquoting working volumes.