Human Phosphotyrosine STAT4 PharmaGenie ELISA Kit (SBRS1989)

The Phosphotyrosine STAT4 ELISA assay from Assay Genie quantitatively measures tyrosine-phosphorylated STAT4 in cell lysates and tissue lysates through a microplate-based immunoassay format. STAT4 is phosphorylated at tyrosine 693 by JAK1 and JAK2 following IL-12 and IL-23 receptor engagement, an event that drives Th1 lineage commitment, IFN-γ expression, and the pathogenic programming of Th17 lymphocytes. Investigators in autoimmune disease research, tumor immunology, and cytokine signaling commonly apply this phospho-specific method to compare basal versus stimulated STAT4 activation across experimental conditions. Each microplate provides reagents sufficient for 96 assays; components should be stored refrigerated upon receipt, with working aliquots frozen for long-term stability, and substrate vials protected from light. Intended for research use only and not validated for clinical diagnostic decision-making.