Human Phosphotyrosine PYK2 PharmaGenie ELISA Kit (SBRS1940)

The Human Phosphotyrosine PYK2 PharmaGenie ELISA Kit from Assay Genie is a phospho-specific immunoassay that quantifies tyrosine-phosphorylated PYK2 (PTK2B) in cell and tissue lysates with human-specific reactivity. PYK2 is a calcium-dependent, non-receptor tyrosine kinase that operates downstream of integrin, G-protein-coupled, and cytokine receptors, governing cytoskeletal remodeling, MAPK cascade activation, and cell migration across immune, neuronal, and vascular systems.

Researchers tracking PYK2 activation in response to stimuli such as glutamate, bradykinin, angiotensin II, or mechanical shear stress pipette 100 uL of prepared sample per well onto the pre-coated microplate. A capture antibody directed against the phosphotyrosine epitope pairs with a conjugated detection antibody to generate a chromogenic signal proportional to phospho-PYK2 abundance, enabling time-course and dose-response analyses of integrin signaling, synaptic plasticity, and focal-adhesion remodeling.

Each kit supplies reagents sufficient for 96 determinations. Upon arrival, unopened components should be stored refrigerated at 4 °C, while reconstituted standards and conjugate are best held at -20 °C in single-use aliquots to minimize freeze-thaw loss. Intended for research use only and not validated for diagnostic applications.