Human Phosphotyrosine PDGFRA PharmaGenie ELISA Kit (SBRS1924)

Phosphotyrosine detection on PDGFRA (platelet-derived growth factor receptor alpha) is a key readout in studies of receptor tyrosine kinase signaling, cell proliferation, and tumor biology, including gastrointestinal stromal tumors and glioblastoma where aberrant PDGFRA activity is clinically relevant. This Assay Genie phosphorylation ELISA targets tyrosine-phosphorylated epitopes on PDGFRA in cell lysates and tissue lysates, with cross-reactivity to mouse and rat PDGFRA orthologs, allowing researchers to quantify receptor activation state rather than total receptor expression. The colorimetric endpoint is read on standard microplate readers, and the assay accommodates lysate inputs prepared with RIPA or NP-40 buffers supplemented with phosphatase inhibitors to preserve the phosphorylation mark.

Recommended workflow includes lysing cells or fresh-frozen tissue in the presence of sodium orthovanadate and protease inhibitors, normalizing total protein concentration before loading, and running a standard curve on the pre-coated plate. Each vial provides sufficient reagents for the indicated number of determinations, and unused wells can be stored sealed with desiccant under refrigerated conditions for short-term use. Researchers studying ligand-induced activation, inhibitor off-target effects, or mutational hyperactivation of PDGFRA will find the assay useful alongside western blot confirmation using phospho-specific antibodies.