Human Phosphotyrosine IGF-1R PharmaGenie ELISA Kit (SBRS1854)

The Insulin-like Growth Factor 1 Receptor (IGF-1R) is a transmembrane receptor tyrosine kinase central to the PI3K/AKT and MAPK signaling cascades, and phosphorylation of its kinase domain tyrosine residues is a key marker of receptor activation. This Phosphotyrosine IGF-1R ELISA from Assay Genie is intended to quantify activated, phosphorylated IGF-1R in cell or tissue lysates prepared from cultured human cell lines or tissue biopsy material, supporting research into IGF-driven proliferation, survival, and oncogenic signaling in tumor biology, metabolic disease, and drug-response studies.

The microplate format provides sufficient wells for replicates, standard curves, and experimental conditions in a single run. Capture antibodies specific to IGF-1R are pre-coated, and a phosphotyrosine-specific detection antibody is used to selectively measure the phosphorylated form of the receptor rather than total receptor expression.

Researchers commonly pair this kit with phosphatase inhibitor cocktails during lysis to preserve phosphorylation, and with Western blot confirmation of receptor activation. The kit is for research use only and is not intended for diagnostic or clinical applications.