Human Phosphotyrosine FGFR1 PharmaGenie ELISA Kit (SBRS1827)

This phosphotyrosine FGFR1 ELISA kit from Assay Genie enables quantitative measurement of activated fibroblast growth factor receptor 1 in cell lysates and tissue lysates. FGFR1 is a receptor tyrosine kinase (~92 kDa) that undergoes autophosphorylation at key tyrosine residues including Tyr653 and Tyr654 upon ligand binding, driving downstream MAPK, PI3K/AKT, and PLCγ signaling cascades. Researchers use this phosphorylation assay to study FGFR1 activation in cancer cell lines, tumor xenografts, and stem cell differentiation models where aberrant FGF signaling contributes to proliferation, angiogenesis, and drug resistance. The microplate format supports up to 96 assays with absorbance readout, suitable for in vitro research on breast, lung, and hematological malignancies. Recommended for research use only, with samples prepared in lysis buffer containing protease and phosphatase inhibitors to preserve phosphorylation status. Store reagents refrigerated and aliquot to limit freeze-thaw cycles that compromise antibody binding activity.