Human Phosphotyrosine FER PharmaGenie ELISA Kit (SBRS1826)

The Human Phosphotyrosine FER PharmaGenie ELISA Kit from Assay Genie enables quantitative measurement of tyrosine-phosphorylated FER kinase in cell and tissue lysate samples. FER (Feline Encephalitis Virus-Related kinase) is a non-receptor tyrosine kinase of the FES/FPS family that regulates cytoskeletal dynamics, cell-cell adhesion, and growth factor signaling pathways, and is frequently investigated in cancer cell signaling, migration, and invasion studies.

This phosphorylation ELISA is supplied as a complete microplate-format kit with pre-coated capture antibodies, recombinant phospho-FER standards, detection reagents, and assay diluent. Researchers typically prepare lysates in RIPA buffer supplemented with protease and phosphatase inhibitors to preserve phosphotyrosine epitopes, loading 100 uL of sample per well. Unused strips should be returned to the foil pouch, sealed, and refrigerated at 4 degrees C between uses, with the kit generally stored at -20 degrees C for long-term stability of the standard and detection antibody components.

Common applications include profiling FER activation in response to EGF or PDGF stimulation, comparing phospho-FER levels across tumor and matched normal tissue, and screening small-molecule kinase inhibitors in vitro. The assay provides a quantitative alternative to Western blot for tracking FER phosphorylation status, with absorbance readings enabling calculation of relative phospho-FER levels against a standard curve.