Human Phosphotyrosine Erb-B3 PharmaGenie ELISA Kit (SBRS1816)

This phosphorylation ELISA from Assay Genie quantifies phosphotyrosine residues on Erb-B3 (HER3), a 180 kDa receptor tyrosine kinase of the ErbB/EGFR family that heterodimerizes with HER2 to drive PI3K/Akt signaling in breast, gastric, and ovarian cancer models.

The kit is supplied as a microplate-based reagent for measuring activated Erb-B3 in cell lysates and tissue lysates prepared from cultured human cells or biopsy material. Researchers investigating trastuzumab resistance, heregulin-mediated signaling, or compensatory PI3K pathway activation use this assay to quantify receptor phosphorylation following ligand stimulation or tyrosine kinase inhibitor treatment.

Each kit contains pre-coated plate wells, detection antibodies, dilution buffers, and substrate sufficient for 96 reactions. Store reagents refrigerated at 4 degrees C prior to use; avoid repeated freeze-thaw cycles of component vials. For research use only; not intended for diagnostic procedures.