Human Phosphotyrosine EphA5 PharmaGenie ELISA Kit (SBRS1808)

The Human Phosphotyrosine EphA5 PharmaGenie ELISA from Assay Genie is a quantitative cell-signaling tool that measures tyrosine-phosphorylated EphA5 in lysates from cultured cells or tissue, supporting research into ephrin receptor biology, axon guidance, and oncology. The microplate format processes up to 96 samples simultaneously using a phospho-specific capture antibody, supporting time-course and dose-response studies of receptor tyrosine kinase activation. Each kit provides pre-coated wells, detection reagents, and a standard curve; researchers typically load 100 µL sample volume per well and incubate at room temperature before adding the substrate. Reagents should be stored refrigerated at 4°C, with the standard aliquoted and frozen at -20°C for repeated freeze-thaw cycles to preserve calibration. The protocol is compatible with downstream western blot validation, allowing orthogonal confirmation of EphA5 phosphorylation status in neuronal, glioma, and prostate cancer models.