Human Phosphotyrosine EphA4 PharmaGenie ELISA Kit (SBRS1807)

The Phosphotyrosine EphA4 PharmaGenie ELISA Kit from Assay Genie enables quantitative measurement of phosphotyrosine on the EphA4 receptor. EphA4 (Ephrin type-A receptor 4) belongs to the Eph receptor tyrosine kinase family and is widely studied for its roles in axon guidance, synaptic plasticity, cell migration, and tissue boundary formation during embryonic development. Detecting tyrosine phosphorylation on EphA4 gives a direct readout of receptor activation following stimulation by ephrin-A or ephrin-B ligands, or modulation by small molecule kinase inhibitors.

The assay is intended for human, mouse, and rat cell lysates and tissue lysates prepared in standard lysis buffer, supporting both in vitro cell culture models and ex vivo tissue specimens. Researchers often pair phospho-EphA4 quantification with western blot analysis to confirm downstream signaling cascades. Each kit includes reagents for 96 assays run in a microplate format. Store reagents according to the enclosed package insert; reconstituted components should be aliquoted to limit freeze-thaw cycles and preserve antibody activity.

Application areas include neuroscience research on axon guidance and neurodegeneration, oncology studies of cancer cell invasion and metastasis, and investigations of bone remodeling, where altered EphA4 phosphorylation has been linked to pathological states. The kit is for research use only and accommodates comparative analyses between treated and untreated samples, dose-response experiments with kinase modulators, and time-course studies of receptor activation following ligand engagement.